General aseptic techniques in microbiology laboratory basic. You have free access to a large collection of materials used in a collegelevel introductory microbiology course. Asepsis is the state of being free from diseasecausing microorganisms such as pathogenic bacteria, viruses, pathogenic fungi, and parasites. Air currents must be controlled by closing laboratory doors and windows to prevent microbes on surfaces from becoming airborne and entering the cultures. Carry out the transfer of cultures as quickly as possible, with tubes and plates open to the air for the minimum length of time. Only nonpathogenic cultures should be used in schools obtained from a recognised educational supplier. Aseptic technique is a procedure used by medical staff to prevent the spread of infection. Refer to your laboratory text for various methods of transferring microbial cultures. Carry out aseptic technique for the removal and transfer of microorganisms for culturing. Aseptic technique microbiology resource center truckee. Aseptic technique and transfer of microorganisms biology.
Techniques for bacteria and fungi fisher scientific. Introduction to microbiology, sterile technique and. Correctly sterilize and flame transfer instruments and tubes. The main document is guidance for aseptic transfer processes in the nhs. Wire loops, also known as inoculation or smear loops, are frequently used in microbiology for the purposes of transfer and. Microbiology is a popular option for practical work in schools. Clean bench area with swab soaked in methylated spirit. Keep in mind that you must wear the correct personal protection equipment ppe in all labs where you are using microbial cultures, stains.
There are also tables of the available products, stability considerations and health and safety issues. Aseptic sterile technique used in microbiology laboratory. Aseptic technique is a fundamental and important laboratory skill in the field of microbiology. Asepsis covers all those procedures designed to reduce the risk of. Aseptic technique is the range of infection prevention and control practices which are used to minimize the presence of pathogenic microorganisms during clinical procedures. The goal is to reach asepsis, which means an environment that is free of harmful microorganisms. Here are some examples of how this technique is practiced. Objectives to demonstrate good aseptic technique in culture transfer or inoculation and in handling sterile materials. All five methods incorporate aseptic technique, or procedures that. Aseptic technique and the transfer of microorganisms. Aseptic technique is absolutely necessary for the successful establishment and maintenance of plant cell, tissue and organ cultures.
All five methods incorporate aseptic technique, or procedures that maintain. Transfer bacterial cells from one plate to another using the. The modern day notion of asepsis is derived from the older antiseptic techniques, a shift initiated by different individuals in the 19th century who introduced practices such as the. You will be provided with staphylococus epidermidis in a broth and on a plate. Nonsterile supplies, media, and reagents, airborne particles laden with microorganisms, unclean incubators, and dirty work surfaces are all sources of biological contamination. Basic practical microbiology why microbiology matters.
Microbiologists use aseptic technique for a variety of procedures such. Choose from 405 different sets of lab microbiology aseptic technique transfer flashcards on quizlet. Transfer bacterial cells from one plate to another using the replicaplating procedure. The in vitro environment in which the plant material is grown is also ideal for the proliferation of microorganisms. General techniques aseptic technique in most microbiological procedures, it is necessary to protect instruments. Sterile aseptic technique michigan state university. Aseptic technique aseptic technique refers to a procedure that is performed under sterile conditions. Aseptic technique is a list of guidelines explaining how to avoid contamination when transferring bacteria from one culture to another. Because bacteria are relatively simple organisms and are amenable to experimental manipulation, they have provided elegant systems for basic research. To acquire the skill of aseptic technique in the field of microbiology. The simplest and most economical way to reduce contamination from airborne particles and aerosols e.
Microbiologists use aseptic technique for a variety of procedures such as transferring cultures, inoculating media, isolation of pure cultures, and for performing microbiological tests. To demonstrate skill in isolation of organisms from a mixed culture using selective and differential media. Manipulation of the tubes, plates and transfer tools requires patience and practice and is vital to success in the microbiology laboratory. Introduction to microbiology, sterile technique and micropipetting background. Aseptic transfer of cultures and sterile solutions 19. Aseptic techniques aseptic technique refers to protocols and procedures you use to insure that no microbes contaminate your. Aseptic technique is a set of best practices that healthcare professionals use to prevent the transfer of germs in clinics and hospitals. The third treatment was to use a nonsterile plate from a previous experiment while following all aseptic techniques to transfer stock bacteria. Common among many experiments in microbiology are techniques involving the measurement and transfer of cultures containing bacterial cells or viral particles. Aseptic transfer technique is important to prevent contamination of the culture being maintained as well as yourself.
Aseptic technique is a set of principles and practices used by cell culture workers to reduce the presence of unwanted microorganisms or other cell lines in their cultures. Replicaplate technique used to transfer cells from primary to secondary plates for. If aseptic techniques are not followed, cultures can become contaminated with nontargeted species of bacteria, causing researchers to study the wrong organism or forcing them to repeat the experiment. A fundamental and important laboratory skill in the field of microbiology. Aseptic technique is a means of performing lab work that greatly reduces the risk of contamination. Aseptic technique and transfer napa valley college. Previously, the terms sterile technique, clean technique and aseptic technique have been used interchangeably. Aseptic transfer of cultures and sterile solutions. Understand the concept of aseptic technique and its importance in the field of microbiology. Aseptic technique steps, purpose and importance of aseptic. Make sure you are familiar with all these techniques before embarking on the other microbiology protocols on this website. Produce nutrient agar na plates, na slants, and nutrient broths nbwhich will be used in subsequent lab periods.
This manual, which explains the basic techniques necessary to carry out microbiology experiments safely and effectively, is intended as a guide for teachers and technicians. It reduces bacterial contamination since the bacteria, even if they get into the plate in. Procedures described include 1 streakplating bacterial cultures to isolate. These will be shared with the table and will be found at the front of each table in a white plastic rack. Aseptic technique in microbiology definition of aseptic.
Sterile work area for labs with cell culture hoods. Become familiar with different forms of basic growth media learn how to transfer a pure bacterial culture from one growth media to another, a. This technique ensures a contaminant free environment while handling micro organisms. How to properly inoculate from broth to broth, broth to slant, and broth to plate tsb, nas, nap duration. Aseptic techniques underpin all work in microbiology. Microbiologist using aseptic techniques to spread bacteria culture on agar. Aseptic technique and the transfer of microorganisms theory. Bacteria can be isolated from nearly any surface including inanimate objects and human skin. An introduction to microbiology, aseptic technique and safety. This method of preventing unwanted microorganisms from gaining access is termed aseptic technique. The microbiology society, in association with the microbiology in schools advisory committee misac. Volume transfers with serological pipettes and micropipettors. Dont touch any part of the container, pipette, etc. Know the laboratory equipment and culture media needed to develop and maintain pure cultures 6.
Asepsis is a condition in which no living diseasecausing microorganisms are present. This includes medical and laboratory techniques which deal with cultures and human cells and tissue for transplantation. Proper aseptic technique prevents contamination of cultures from foreign bacteria inherent in the environment. Asepsis is the state of remaining free from pathogenic and contaminating microorganisms. Microbiological laboratory techniques clinical gate. This manual has been written for a righthanded person. In the microbiology lab we use aseptic technique to.
Use pour plating and spreadplating methods to determine the concentration of bacteria. Laboratory safety adapted from the american society of microbiology website. All five methods incorporate aseptic technique, or procedures that maintain the sterility of experimental materials. General aseptic techniques in microbiology laboratory. An aseptic technique experiment was conducted to test the effects of aseptic technique on the transfer of target bacteria, sarcina aurantiaca s. Bacteria represent the most widely distributed and metabolically diverse group of organisms.
Aseptic technique an overview sciencedirect topics. The swab method a swab sample is taken from colonies to ensure the use of proper techniques and sterilization. Aseptic technique prevents contamination of sterile substances or objects two common isolation techniques streak plates pour plates. In the context of medicine, aseptic techniques are used by medical staff to prevent the spread of infection, or to rid the environment of pathogenic microorganisms. Learn lab microbiology aseptic technique transfer with free interactive flashcards.
To transfer bacteria, yeasts, or fungi with abundant spores, place the stock. Experiment 1 culture transfer technique purpose technique of aseptic removal and transfer of microorganisms for subculturing principle microorganisms are transferred from one medium to another by subculturing. Successful cell culture depends heavily on keeping the cells free from contamination by microorganisms such as bacterial, fungi, and viruses. Christie t ammirati md, in surgery of the skin, 2005. Aseptic technique is a set of routine measures that are taken to prevent cultures, sterile media stocks, and other solutions from being contaminated by unwanted microorganisms i. General microbiology bio3302 syllabus new york city college of technology the city university of new york. Aseptic technique is essential in the microbiology laboratory to prevent any contamination of laboratory personnel see chapter 5, safety issues, cultures, supplies, and equipment. To prevent contamination of cultures and media from microbes in the environment. The aseptic techniques control the opportunities for contamination of. This yellow cover document explains the need, outlines the risks and points the way to best practice. An introduction to microbiology, aseptic technique and safety preparation safety guidelines 1 risk assessment 2 good microbiological laboratory practice gmlp 3 spillage management 3 aerosols 3 resources equipment 4 apparatus 5 materials 5 media, sterilisation and disinfection preparation of culture media 6 pouring a plate 6 storage of media 6.
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